Study of Tannase from Aspergillus niger
Author Information
Author(s): Abril Flores-Maltos, Luis V. Rodríguez-Durán, Jacqueline Renovato, Juan C. Contreras, Raúl Rodríguez, Cristóbal N. Aguilar
Primary Institution: Autonomous University of Coahuila
Hypothesis
The immobilization of tannase from Aspergillus niger will enhance its catalytic properties compared to the free enzyme.
Conclusion
Immobilized tannase from Aspergillus niger is a better biocatalyst for applications in liquid systems with high tannin content.
Supporting Evidence
- Tannase was produced intracellularly by Aspergillus niger in submerged fermentation.
- Immobilization in calcium alginate beads improved the enzyme's stability and recovery.
- The study showed that immobilized tannase had higher K_M and V_max values compared to the free enzyme.
Takeaway
Scientists found a way to trap an enzyme called tannase in beads, making it work better for cleaning up waste from tanneries.
Methodology
Tannase was produced by submerged fermentation, recovered, and immobilized in calcium alginate beads; its catalytic properties were compared using different substrates.
Limitations
The study's findings may not apply to all types of tannase or fermentation methods.
Digital Object Identifier (DOI)
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