Posttranslational N-glycosylation of the hepatitis B virus large envelope protein
2007
Posttranslational N-glycosylation of Hepatitis B Virus Large Envelope Protein
publication
Evidence: moderate
Author Information
Author(s): Carsten Lambert, Reinhild Prange
Primary Institution: Institute for Medical Microbiology and Hygiene, University of Mainz
Hypothesis
Can the hepatitis B virus large envelope protein undergo posttranslational N-glycosylation?
Conclusion
The posttranslational N-glycosylation of the hepatitis B virus large envelope protein is not necessary for virus maturation.
Supporting Evidence
- The study identified novel isoforms of the HBV L protein due to posttranslational N-glycosylation.
- Inhibition of N-glycosylation blocked virus assembly and release.
- Posttranslational N-glycosylation of L was found to be dispensable for HBV morphogenesis.
Takeaway
This study found that a part of the hepatitis B virus protein can get sugar chains added to it after it's made, but this doesn't help the virus to grow.
Methodology
The study used mutational analyses, N-glycosylation inhibitors, and pulse-chase studies to investigate the glycosylation of the HBV L protein.
Digital Object Identifier (DOI)
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