Oxidized lipoproteins suppress nitric oxide synthase in macrophages
Author Information
Author(s): K. E. Matthys, P. G. Jorens, B. Marescau, M. Rosseneu, H. Bult, A. G. Herman
Primary Institution: University of Antwerp
Hypothesis
The study investigates whether a glucocorticoid-like factor formed during the oxidation of LDL inhibits nitric oxide synthase activity in macrophages.
Conclusion
The study found that nitric oxide synthase activity is specifically inhibited in macrophages loaded with oxidized low-density lipoproteins, but not in those loaded with acetylated low-density lipoproteins.
Supporting Evidence
- Oxidized low-density lipoproteins caused a small induction of nitric oxide synthase activity in unstimulated cells.
- Oxidized low-density lipoproteins suppressed nitric oxide synthase activity in stimulated macrophages.
- Acetylated low-density lipoproteins did not inhibit nitric oxide synthase activity.
- The glucocorticoid receptor antagonist mifepristone did not prevent the inhibition of nitric oxide synthase by oxidized low-density lipoproteins.
Takeaway
When certain fats in the blood get oxidized, they can stop immune cells from making a helpful chemical called nitric oxide, which is important for fighting off infections.
Methodology
The study involved loading J774 macrophages with oxidized or acetylated low-density lipoproteins and measuring nitric oxide synthase activity after stimulation with lipopolysaccharide.
Limitations
The exact mechanism of inhibition by oxidized lipoproteins remains unclear.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Want to read the original?
Access the complete publication on the publisher's website