Effect of Trinitrobenzene Sulfonic Acid on Gut Muscle Cells
Author Information
Author(s): W. E. Longo, G. S. Smith, Y. Deshpande, C. Reickenberg, D. L. Kaminski
Primary Institution: St. Louis University School of Medicine and Health Sciences Center
Hypothesis
The study investigates the effect of trinitrobenzene sulfonic acid on eicosanoid production in gut-derived smooth muscle cells.
Conclusion
Gut-derived smooth muscle cells may be a significant source of pro-inflammatory prostanoids during intestinal inflammation.
Supporting Evidence
- Incubation with TNB led to significant increases in PGE2 and 6-keto PGF-1a release.
- Responses were inhibited by dimethylthiourea and indomethacin treatment.
- Thiourea may inhibit prostanoid activity by suppressing cyclooxygenase activity.
Takeaway
This study found that certain cells in the gut can produce substances that cause inflammation when exposed to a specific chemical.
Methodology
Human gut-derived smooth muscle cells were incubated with trinitrobenzene sulfonic acid and treated with inhibitors to assess eicosanoid production.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
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