Identification of Novel Biomarkers in Seasonal Allergic Rhinitis by Combining Proteomic, Multivariate and Pathway Analysis
2011

Identifying Biomarkers for Allergic Rhinitis Treatment

Sample size: 40 publication 10 minutes Evidence: moderate

Author Information

Author(s): Wang Hui, Gottfries Johan, Barrenäs Fredrik, Benson Mikael

Primary Institution: University of Gothenburg

Hypothesis

Proteins that correlate with the discrimination between high and low responders to glucocorticoid treatment may serve as biomarkers for treatment response in seasonal allergic rhinitis.

Conclusion

Several novel biomarkers for glucocorticoid treatment response in seasonal allergic rhinitis were identified using combined proteomics, multivariate, and pathway analysis.

Supporting Evidence

  • 953 unique proteins were identified in nasal fluids from patients with seasonal allergic rhinitis.
  • The acute phase response pathway was significantly enriched in the identified proteins.
  • ORM, FGA, and APOH decreased significantly in high responders after glucocorticoid treatment.

Takeaway

Researchers found new proteins in nasal fluids that can help predict how well people with allergies will respond to treatment.

Methodology

The study used liquid chromatography-tandem mass spectrometry (LC-MS/MS) for proteomic analysis and ELISA for validation of candidate biomarkers.

Potential Biases

Potential bias due to individual variations in treatment response and compliance.

Limitations

The study had a relatively small sample size and the proteomics analysis may miss low-abundance proteins.

Participant Demographics

Median age of participants was 23 years, with 24 women included.

Statistical Information

P-Value

P<0.0001

Confidence Interval

99%

Statistical Significance

p<0.0001

Digital Object Identifier (DOI)

10.1371/journal.pone.0023563

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