Maintaining Cell Types in Human Brain Tissue Cultures
Author Information
Author(s): McGinnis JP, Ortiz-Guzman Joshua, Mallannagari Sai, Guevara Maria Camila, Belfort Benjamin D. W., Bao Suyang, Srivastava Snigdha, Morkas Maria, Ji Emily, Katlowitz Kalman A., Addison Angela, Tantry Evelyne K., Blessing Melissa M., Mohila Carrie A., Gadgil Nisha, McClugage Samuel G., Bauer David F., Whitehead William E., Aldave Guillermo, Tanweer Omar, Jaleel Naser, Jalali Ali, Patel Akash J., Sheth Sameer A., Weiner Howard L., Gopinath Shankar, Rao Ganesh, Harmanci Akdes Serin, Curry Daniel, Arenkiel Benjamin R.
Primary Institution: Cold Spring Harbor Laboratory
Hypothesis
Can the transcriptional identities of various cell types in human brain tissue be maintained in organotypic cultures?
Conclusion
The study found that transcriptional identities of various cell types in human brain tissue are largely preserved after 14 days in culture.
Supporting Evidence
- The study analyzed 83,501 nuclei from day 0 and 45,738 nuclei from day 14 samples.
- High correlations were found in transcriptional identities of tumor cells between day 0 and day 14.
- Microglia, oligodendrocytes, pericytes, endothelial cells, and fibroblasts showed strong preservation of their transcriptional profiles.
Takeaway
Scientists are trying to grow human brain cells in a lab, and they found that most types of brain cells stay the same even after two weeks.
Methodology
Single-nucleus RNA sequencing was used to evaluate transcriptional identities in brain tissue samples before and after 14 days in culture.
Limitations
The study is limited to a small number of patient samples and specific brain regions.
Participant Demographics
Included pediatric and adult patients with various brain conditions.
Digital Object Identifier (DOI)
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