How PAR1 Activation Affects Neuron Excitability in the Hippocampus
Author Information
Author(s): Han Kyung-Seok, Mannaioni Guido, Hamill Cecily E, Lee Jaekwang, Junge Candice E, Lee C Justin, Traynelis Stephen F
Primary Institution: Department of Pharmacology, Emory University School of Medicine
Hypothesis
Does activation of protease activated receptor 1 (PAR1) increase the excitability of dentate granule neurons in the hippocampus?
Conclusion
Activation of PAR1 increases the excitability of dentate granule cells, enhancing their action potential generation and NMDA receptor-mediated synaptic transmission.
Supporting Evidence
- PAR1 activation increased intracellular Ca2+ concentration in dentate neurons.
- Activation of PAR1 by TFLLR depolarized the dentate gyrus in wild type but not in PAR1-/- mice.
- PAR1 activation enhanced the frequency of action potential generation in dentate granule neurons.
- PAR1 activation increased the amplitude of NMDA receptor-mediated EPSPs.
Takeaway
When a specific receptor in the brain is turned on, it makes certain brain cells more active, which can help them send signals better.
Methodology
The study used immunohistochemistry, Ca2+ imaging, whole-cell patch clamp recording, and voltage-sensitive dye imaging to assess the effects of PAR1 activation on dentate granule neurons.
Participant Demographics
Wild type C57Bl/6 mice were used in the experiments.
Statistical Information
P-Value
p < 0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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