HIV-1 R5 Variants and Their Sensitivity to Entry Inhibitors
Author Information
Author(s): Paul J Peters, Maria J Duenas-Decamp, W Matthew Sullivan, Richard Brown, Chiambah Ankghuambom, Katherine Luzuriaga, James Robinson, Dennis R Burton, Jeanne Bell, Peter Simmonds, Jonathan Ball, Paul R Clapham
Primary Institution: University of Massachusetts Medical School
Hypothesis
How does variation in HIV-1 R5 macrophage-tropism affect sensitivity to entry inhibitors and neutralizing antibodies?
Conclusion
Variation in R5 macrophage-tropism is influenced by envelope variation that primarily affects sensitivity to reagents blocking gp120:CD4 interactions.
Supporting Evidence
- R5 macrophage-tropism correlated with increased sensitivity to soluble CD4 and PRO 542.
- Macrophage-tropic envelopes were more resistant to the anti-CD4 monoclonal antibody Q4120.
- No overall correlations were noted between R5 macrophage-tropism and sensitivity to CCR5 antagonists.
Takeaway
Some types of HIV can infect certain cells better than others, and this affects how well treatments work against them.
Methodology
The study analyzed the sensitivity of 36 R5 envelopes derived from patient samples to various entry inhibitors and neutralizing antibodies.
Potential Biases
Potential bias due to the selection of envelopes from specific tissue types.
Limitations
The study focused only on R5 envelopes and did not include R5X4 or X4 envs.
Participant Demographics
Envelopes were derived from various tissues including brain, lymph node, blood, and semen from HIV-infected patients.
Statistical Information
P-Value
<0.0001
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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