Monitoring Growth of Human Embryonic Stem Cells
Author Information
Author(s): Susanna Narkilahti, Kristiina Rajala, Harri Pihlajamäki, Riitta Suuronen, Outi Hovatta, Heli Skottman
Primary Institution: REGEA, Institute for Regenerative Medicine, University of Tampere and Tampere University Hospital
Hypothesis
Can automated systems improve the monitoring and analysis of human embryonic stem cell growth compared to conventional methods?
Conclusion
The automated system allows for rapid and reliable analysis of undifferentiated growth dynamics of human embryonic stem cells.
Supporting Evidence
- The automated system allowed continuous monitoring of hESC colony growth.
- Colony growth was significantly better in conventional medium than in X-vivo 10 medium.
- The average size of colonies in X-vivo 10 medium was smaller than in conventional medium.
Takeaway
Scientists used a new machine to watch how stem cells grow without bothering them, and found that some growing conditions worked better than others.
Methodology
The study compared two hESC lines grown in conventional and xeno-free media using an automated culture platform and validated results with flow cytometry.
Limitations
The study did not explore the application of the automated system to 3D cell differentiation.
Participant Demographics
Human embryonic stem cell lines HS237 and HS293 were used.
Statistical Information
P-Value
<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website