Identification, Replication, and Functional Fine-Mapping of Expression Quantitative Trait Loci in Primary Human Liver Tissue
2011

Identifying Genetic Factors Affecting Liver Gene Expression

Sample size: 206 publication 10 minutes Evidence: moderate

Author Information

Author(s): Innocenti Federico, Cooper Gregory M., Stanaway Ian B., Gamazon Eric R., Smith Joshua D., Mirkov Snezana, Ramirez Jacqueline, Liu Wanqing, Lin Yvonne S., Moloney Cliona, Aldred Shelly Force, Trinklein Nathan D., Schuetz Erin, Nickerson Deborah A., Thummel Ken E., Rieder Mark J., Rettie Allan E., Ratain Mark J., Cox Nancy J., Brown Christopher D.

Primary Institution: The University of Chicago

Hypothesis

Can we identify genetic determinants of human liver gene expression variation?

Conclusion

The study found that many eQTLs are reproducible and linked to complex traits, with significant functional variants identified.

Supporting Evidence

  • Approximately 30% of SNP-expression correlations failed to replicate across studies.
  • Reproducible eQTL SNPs were enriched near gene starts and ends.
  • Three genes showed significant haplotype-specific functional differences correlated with expression levels.

Takeaway

Scientists looked at how genes in the liver are turned on or off by different genetic factors, finding many important links to diseases.

Methodology

The study used two independent collections of primary liver tissues and performed genotype imputation and regression analyses.

Potential Biases

Potential biases due to unmeasured confounding variables and differences in sample collection methods.

Limitations

The study's conclusions may be affected by sample size differences and the use of different expression platforms.

Participant Demographics

The study included liver samples from individuals of self-reported European and African descent.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pgen.1002078

Want to read the original?

Access the complete publication on the publisher's website

View Original Publication