Expression of the Multiple Sclerosis-Associated MHC Class II Allele HLA-DRB1*1501 Is Regulated by Vitamin D
2009

Vitamin D and Multiple Sclerosis Risk

Sample size: 490 publication 10 minutes Evidence: high

Author Information

Author(s): Ramagopalan Sreeram V., Maugeri Narelle J., Handunnetthi Lahiru, Lincoln Matthew R., Orton Sarah-Michelle, Dyment David A., DeLuca Gabriele C., Herrera Blanca M., Chao Michael J., Sadovnick A. Dessa, Ebers George C., Knight Julian C.

Primary Institution: Wellcome Trust Centre for Human Genetics, University of Oxford

Hypothesis

Does vitamin D interact with inherited factors to influence the expression of the HLA-DRB1 gene associated with multiple sclerosis?

Conclusion

The study demonstrates that vitamin D directly interacts with the HLA-DRB1 gene, which is a major genetic risk factor for multiple sclerosis.

Supporting Evidence

  • Vitamin D receptor was specifically recruited to the HLA-DRB1 promoter in the presence of vitamin D.
  • Significant increase in HLA-DRB1 expression was observed upon vitamin D stimulation in specific cell lines.
  • Conservation of the vitamin D response element was found in MS-associated haplotypes.

Takeaway

This study found that vitamin D helps control a gene linked to multiple sclerosis, suggesting that getting enough vitamin D might help prevent the disease.

Methodology

The study involved sequencing the HLA-DRB1 promoter in MS patients and controls, in vitro binding assays, and flow cytometry to assess gene expression.

Potential Biases

Potential bias due to the specific population studied and the reliance on self-reported data for vitamin D intake.

Limitations

The study's findings may not be generalizable beyond Northern European populations.

Participant Demographics

Participants included individuals of Northern European descent, both MS affected and unaffected.

Statistical Information

P-Value

0.002

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pgen.1000369

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