Jak2-Independent Activation of Stat3 by Intracellular Angiotensin II in Human Mesangial Cells
2011
How Intracellular Angiotensin II Affects Kidney Cells
Sample size: 5
publication
10 minutes
Evidence: moderate
Author Information
Author(s): Rekha Singh
Primary Institution: Hines VA Medical Center
Hypothesis
Can intracellular Ang II stimulate TGF-b1 and matrix proteins independently of extracellular Ang II?
Conclusion
Intracellular Ang II increases TGF-b1 and matrix proteins in human mesangial cells and activates Stat3 without involving the extracellular Ang II signaling pathway.
Supporting Evidence
- Transfection of mesangial cells with Ang II increased intracellular Ang II levels significantly.
- Increased intracellular Ang II levels led to higher production of TGF-b1, collagen IV, and fibronectin.
- Stat3 phosphorylation at tyrosine 705 was significantly increased by intracellular Ang II.
Takeaway
This study shows that a substance called Ang II inside kidney cells can make them grow and produce more proteins, even when the outside signals are blocked.
Methodology
Human mesangial cells were transfected with Ang II to increase intracellular levels, while extracellular signaling was blocked with candesartan.
Participant Demographics
Primary normal human mesangial cells were used.
Statistical Information
P-Value
<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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