DNA Aptamer VZ23 Inhibits FGFR1 Signaling
Author Information
Author(s): Zlinska Vladimira, Feketova Zuzana, Czyrek Aleksandra, Chudzian Julia, Zivkovic Martina Lenarcic, Ursachi Vlad-Constantin, Dudeja Pooja, Fafilek Bohumil, Rynes Jan, Rico-Llanos Gustavo, Koudelka Adolf, Roy Tanaya, Biadun Martyna, Raskova Vendula, Svozilova Katerina, Stroblova Michaela, Krzyscik Mateusz, Hristova Kalina, Krowarsch Daniel, Foldynova-Trantirkova Silvie, Zakrzewska Malgorzata, Trantirek Lukas, Krejci Pavel
Primary Institution: Masaryk University
Hypothesis
Can a DNA aptamer specifically inhibit FGFR1 signaling?
Conclusion
The DNA aptamer VZ23 effectively inhibits FGFR1 signaling without affecting other FGFR variants.
Supporting Evidence
- VZ23 binds specifically to FGFR1b and FGFR1c with KD values of 55 nM and 162 nM, respectively.
- VZ23 does not inhibit signaling of FGFR2, FGFR3, or FGFR4.
- VZ23's inhibitory effect on FGFR1 signaling was observed in multiple cell types.
Takeaway
Scientists created a special DNA piece that can stop a specific signal in cells that can cause problems like cancer. This could help treat certain diseases better.
Methodology
The study used SELEX to identify the DNA aptamer VZ23, which was tested for its ability to inhibit FGFR1 signaling in various cell models.
Limitations
The study primarily focused on in vitro models, and further research is needed to assess the efficacy of VZ23 in vivo.
Statistical Information
P-Value
0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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