TYMS Enhances Colorectal Cell Antioxidant Capacity Via the KEAP1-NRF2 Pathway to Resist Ferroptosis
Author Information
Author(s): Chen Jingtian, Wu Wei, Wang Lingxiao, Zhang Jingjing, Che Xin, Zhai Liqin, Zhao Zhenxiang, Li Yaoping
Primary Institution: Shanxi Medical University
Hypothesis
TYMS enhances the antioxidant capacity of colorectal cancer cells through the KEAP1-NRF2 pathway, increasing resistance to ferroptosis.
Conclusion
TYMS was overexpressed in colorectal cancer, which was correlated with poor prognosis, and it enhanced the antioxidant capacity of CRC cells via the KEAP1-NRF2 pathway, thereby increasing resistance to ferroptosis.
Supporting Evidence
- TYMS expression was upregulated in CRC tumor tissues compared to adjacent non-cancerous tissues.
- Overexpression of TYMS was associated with enhanced cellular antioxidant capacity.
- TYMS knockdown sensitized CRC cells to erastin-induced ferroptosis.
- High TYMS expression correlated with poor prognosis in CRC patients.
- TYMS overexpression promoted NRF2 translocation into the nucleus.
Takeaway
This study found that a protein called TYMS helps cancer cells survive by making them better at fighting off damage, which can help them resist a type of cell death called ferroptosis.
Methodology
The study used western blot, immunohistochemistry, MTT assays, colony formation assays, and xenograft models to assess TYMS expression and its effects on CRC cells.
Limitations
The study's findings may be limited by the sample size and the lack of statistical significance in some correlations.
Participant Demographics
The study included 173 CRC patients, with 97 males and 76 females, aged 32 to 85 years, and a median age of 62 years.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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