Carbon Monoxide Dehydrogenase Diversity in the Homoacetogenic Hindgut Microbial Communities of Lower Termites and the Wood Roach
2011

Diversity of Anaerobic Carbon Monoxide Dehydrogenase in Termite Gut Microbes

Sample size: 151 publication 10 minutes Evidence: moderate

Author Information

Author(s): Eric G. Matson, Kasia G. Gora, Jared R. Leadbetter

Primary Institution: California Institute of Technology

Hypothesis

What is the diversity of anaerobic carbon monoxide dehydrogenase (CODH) genes in the gut microbial communities of lower termites and wood roaches?

Conclusion

The study found a diverse range of CODH genes in the gut microbiota of various insect species, indicating different microbial species participate in acetogenesis.

Supporting Evidence

  • The primers developed can amplify a broad diversity of cooS genes.
  • 151 different cooS genes were sequenced and analyzed.
  • Each insect gut community contained CODH variants from three distinct phylogenetic clades.

Takeaway

Scientists studied tiny bugs' stomachs to see how many different types of a special enzyme they had that helps them eat wood. They found lots of different kinds!

Methodology

The researchers developed degenerate primers to amplify cooS genes from gut bacteria and sequenced the resulting products to analyze diversity.

Limitations

The study may have primer bias affecting the amplification of certain cooS genes over others.

Participant Demographics

The study focused on gut microbial communities from three species of lower termites and two species of cockroaches.

Digital Object Identifier (DOI)

10.1371/journal.pone.0019316

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