Human respiratory organoids help study rhinovirus C
Author Information
Author(s): Li Cun, Yu Yifei, Wan Zhixin, Chiu Man Chun, Huang Jingjing, Zhang Shuxin, Zhu Xiaoxin, Lan Qiaoshuai, Deng Yanlin, Zhou Ying, Xue Wei, Yue Ming, Cai Jian-Piao, Yip Cyril Chik-Yan, Wong Kenneth Kak-Yuen, Liu Xiaojuan, Yu Yang, Huang Lin, Chu Hin, Chan Jasper Fuk-Woo, Clevers Hans, Yuen Kwok Yung, Zhou Jie
Primary Institution: The University of Hong Kong
Hypothesis
We hypothesized that respiratory organoids may be susceptible to HRV-C and sustain virus cultivation.
Conclusion
The study developed an organoid-based system to propagate HRV-C and characterized its infection in respiratory organoids.
Supporting Evidence
- Airway organoids were inoculated with HRV-C positive clinical specimens, showing increased viral load.
- Nasal organoids sustained serial propagation of HRV-C without any intervention.
- CYT387 treatment enabled serial HRV-C passage in airway organoids.
- RNA sequencing revealed a stronger innate immune response in airway organoids than in nasal organoids.
- CDHR3 was identified as the receptor for HRV-C in both airway and nasal organoids.
Takeaway
Scientists created tiny lung models to grow a cold virus that usually can't be grown in labs, helping us understand how it works.
Methodology
The study used human respiratory organoids to propagate HRV-C and assess virus-host interactions.
Limitations
The study primarily focused on HRV-C and may not generalize to other viruses.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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