Real-time assessment of mitochondrial function in C. elegans
Author Information
Author(s): Lagido Cristina, Pettitt Jonathan, Flett Aileen, Glover L Anne
Primary Institution: University of Aberdeen
Hypothesis
Whole animal luminescence reflects its intracellular ATP levels in vivo.
Conclusion
C. elegans luminescence reports on ATP levels in real-time, enabling genetic analysis based on a quantifiable metabolic phenotype.
Supporting Evidence
- Bioluminescence decreased with increasing sublethal azide levels.
- Worms fully recovered luminescence after removal from azide.
- RNA interference of respiratory chain genes decreased luminescence consistent with ATP levels.
- Luciferase expression levels did not influence final luminescence.
Takeaway
Scientists made worms that glow to see how much energy they have. When the worms are stressed, they glow less, showing they have less energy.
Methodology
C. elegans were modified to express firefly luciferase for real-time ATP level assessment during exposure to sodium azide and RNA interference.
Limitations
The exact relationship between light output and ATP concentrations may vary under different experimental conditions.
Participant Demographics
C. elegans nematodes were used in the study.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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