Rotavirus Infection and the Unfolded Protein Response
Author Information
Author(s): Zambrano Jose Luis, Ettayebi Khalil, Maaty Walid S, Faunce Nicholas R, Bothner Brian, Hardy Michele E
Primary Institution: Montana State University
Hypothesis
The study aims to identify changes in the cellular proteome in response to rotavirus infection in the context of the IFN response.
Conclusion
Rotavirus infection activates the unfolded protein response (UPR) but modulates its effects by sequestering key proteins in viroplasms.
Supporting Evidence
- Rotavirus infection leads to changes in the levels of multiple proteins associated with cellular stress.
- GRP78 and GRP94 were found to be localized to viroplasms in infected cells.
- Activation of the UPR was indicated by the presence of phosphorylated PERK and spliced XBP1 mRNA.
Takeaway
When rotavirus infects cells, it triggers a stress response, but then hides important proteins that help fight the infection, making it harder for the body to respond.
Methodology
2D-DIGE and immunofluorescence microscopy were used to analyze protein expression changes in response to rotavirus infection and IFN treatment.
Limitations
The study primarily focuses on a specific strain of rotavirus and may not generalize to all strains.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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