E-cadherin's Role in Mouse Embryonic Stem Cells
Author Information
Author(s): Francesca Soncin, Lisa Mohamet, Sarah Ritson, Kate Hawkins, Nicoletta Bobola, Leo Zeef, Catherine L. R. Merry, Christopher M. Ward
Primary Institution: The University of Manchester
Hypothesis
E-cadherin is required for LIF-dependent pluripotency of mouse embryonic stem cells.
Conclusion
Loss of E-cadherin in mouse embryonic stem cells leads to significant transcript alterations independently of β-catenin/TCF transactivation.
Supporting Evidence
- E-cadherin null ES cells maintain expression of pluripotency markers via Activin/Nodal signalling.
- Only 2 genes showed statistically significant differences in transcript expression in the absence of LIF.
- Ecad-/- ES cells exhibit increased proliferation compared to wtD3 ES cells.
Takeaway
E-cadherin helps keep stem cells in a special state where they can become any type of cell, and without it, the cells change a lot.
Methodology
Global transcript expression was assessed in E-cadherin null ES cells cultured with or without LIF and compared to the parental cell line.
Limitations
The study primarily focuses on transcript alterations without exploring functional outcomes in detail.
Statistical Information
P-Value
<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website