Low-risk susceptibility alleles in breast cancer cell lines
Author Information
Author(s): Riaz Muhammad, Elstrodt Fons, Hollestelle Antoinette, Dehghan Abbas, Klijn Jan GM, Schutte Mieke
Primary Institution: Erasmus University Medical Center
Hypothesis
Do low-risk SNPs modulate expression levels of nearby located genes in breast cancer cell lines?
Conclusion
The study found no evidence that low-risk SNPs modulate gene expression in breast cancer cell lines.
Supporting Evidence
- The SNP genotypes provide a baseline for functional studies in breast cancer cell lines.
- Expression analyses suggest that gene expression modulation is not operative in breast cancer cell lines.
- Minor allele frequencies among the cell lines were higher than in breast cancer cases and population controls.
Takeaway
The researchers looked at genes related to breast cancer but found that the genetic variations they studied didn't change how those genes worked in the lab.
Methodology
Genotypes of five low-risk SNPs were determined by direct sequencing, and gene expression was analyzed using real-time RT-PCR and microarrays.
Limitations
The study's findings may not apply to actual breast cancer patients as it was conducted in cell lines.
Statistical Information
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website