Prosecutor: A Tool for Inferring Gene Function in Prokaryotes
Author Information
Author(s): Evert Jan Blom, Rainer Breitling, Klaas Jan Hofstede, Jos BTM Roerdink, Sacha AFT van Hijum, Oscar P Kuipers
Primary Institution: University of Groningen
Hypothesis
Can DNA microarray data and genomic context help predict gene functions in prokaryotes?
Conclusion
Prosecutor software allows researchers to quickly identify the putative functions of genes in various prokaryotic organisms.
Supporting Evidence
- Prosecutor can analyze publicly available transcriptome datasets.
- The software provides a parameter-free approach to functional prediction.
- It utilizes genomic context and regulatory mechanisms specific to prokaryotes.
Takeaway
Prosecutor is a computer program that helps scientists figure out what genes do by looking at how they behave in different situations.
Methodology
Prosecutor uses DNA microarray datasets and functional categories from various annotation sources to infer gene functions through an iterative guilt-by-association method.
Potential Biases
Potential bias may arise from the reliance on existing annotations and datasets.
Limitations
The software's predictions are based on available data, which may not cover all genes or conditions.
Statistical Information
P-Value
2e-16
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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