The Role of TRAPPC2 in TRAPP Complex Formation and Associated Diseases
Author Information
Author(s): Zong Min, Wu Xing-gang, Chan Cecilia W. L., Choi Mei Y., Chan Hsiao Chang, Tanner Julian A., Yu Sidney
Primary Institution: The Chinese University of Hong Kong
Hypothesis
How do TRAPPC2 and its mutations affect the formation of TRAPPII and TRAPPIII complexes in mammalian cells?
Conclusion
TRAPPC2 acts as an adaptor for the formation of TRAPPII and TRAPPIII complexes, and mutations in TRAPPC2 can impair this function, leading to associated diseases.
Supporting Evidence
- TRAPPC2 binds to TRAPPC9 and TRAPPC8, facilitating the formation of TRAPPII and TRAPPIII complexes.
- Mutations in TRAPPC2, such as D47Y, significantly reduce its ability to interact with TRAPPC9 and TRAPPC8.
- TRAPPC9 mutations lead to reduced binding to TRAPPC2 and TRAPPC10, affecting complex formation.
Takeaway
TRAPPC2 helps build important protein complexes in our cells, and when it has mistakes in its code, it can lead to health problems.
Methodology
Co-immunoprecipitation experiments were conducted to analyze the interactions between TRAPPC2 and other TRAPP subunits in mammalian cells.
Limitations
The study primarily focuses on in vitro interactions and may not fully represent in vivo conditions.
Digital Object Identifier (DOI)
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