Combined Rapid Test for Typhoid and Paratyphoid A Fever
Author Information
Author(s): Yan Meiying, Tam Frankie C. H., Kan Biao, Lim Pak Leong
Primary Institution: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention
Hypothesis
Can a combined rapid test effectively detect both typhoid and paratyphoid A fever using monoclonal antibodies?
Conclusion
The combined TUBEX test demonstrated higher sensitivity for detecting typhoid and paratyphoid A fever compared to traditional methods.
Supporting Evidence
- The combined TUBEX test showed 100% sensitivity for typhoid in early disease stages.
- TUBEX 12T detected more cases than traditional methods.
- High concordance was observed between TUBEX and ELISA results.
- Significant differences in sensitivity were noted between typhoid and paratyphoid tests.
- Specimen quality significantly affected test sensitivity.
Takeaway
This study created a new test that can quickly tell if someone has typhoid or paratyphoid A fever, which are similar diseases.
Methodology
The study used monoclonal antibodies in a colorimetric immunoassay to detect specific antibodies in patient sera.
Potential Biases
Potential bias due to the reliance on culture-confirmed cases for sensitivity assessment.
Limitations
The study's findings may be limited by the quality of stored serum samples and the specificity of the tests.
Participant Demographics
Typhoid patients aged 7 to 19 years and paratyphoid A patients aged 15 to 64 years.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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