Blood Biomarker Signature for Diagnosing Septicemic Melioidosis
Author Information
Author(s): Pankla Rungnapa, Buddhisa Surachat, Berry Matthew, Blankenship Derek M, Bancroft Gregory J, Banchereau Jacques, Lertmemongkolchai Ganjana, Chaussabel Damien
Primary Institution: Khon Kaen University
Hypothesis
Can genomic transcriptional profiling identify a blood biomarker signature for diagnosing septicemic melioidosis?
Conclusion
A blood transcriptional signature can distinguish patients with septicemic melioidosis from those with sepsis caused by other pathogens.
Supporting Evidence
- Microarray analysis identified a 37 transcript candidate diagnostic signature.
- The diagnostic signature showed 100% accuracy in a training set.
- Validation sets confirmed high prediction accuracies of 78% and 80%.
- The signature was enriched in genes involved in MHC class II antigen processing.
Takeaway
Researchers found a special pattern in the blood of people with a serious infection called melioidosis that can help doctors tell it apart from other infections.
Methodology
Microarray technology was used to analyze blood samples from patients with septicemic melioidosis, other sepsis, and uninfected controls.
Potential Biases
Potential bias due to the small sample size and specific population studied.
Limitations
The study's findings need confirmation in larger trials.
Participant Demographics
The study included 598 subjects, primarily Asian, with 32 patients diagnosed with septicemic melioidosis.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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