Interaction between Ku80 protein and APC antibody
Author Information
Author(s): Roberts G T, Davies M L, Wakeman J A
Primary Institution: School of Biological Sciences, University of Wales Bangor
Hypothesis
Does the APC(N15) antibody interact with the Ku80 protein?
Conclusion
The APC(N15) antibody shows a strong nonspecific interaction with the Ku80 subunit, making it unsuitable for its intended use.
Supporting Evidence
- The APC(N15) antibody was shown to interact strongly with the Ku80 protein.
- Immunoprecipitation experiments revealed that APC(N15) pulled down Ku80 along with other proteins.
- Mass spectrometry confirmed the identity of the proteins interacting with APC(N15).
Takeaway
The APC(N15) antibody, which is supposed to detect a protein called APC, actually sticks to another protein called Ku80 instead.
Methodology
The study used immunoprecipitation, Western blotting, and mass spectrometry to analyze protein interactions.
Potential Biases
Potential bias due to reliance on a single antibody for conclusions about APC detection.
Limitations
The study primarily focuses on one antibody and its interactions, which may not represent all antibodies used for APC detection.
Digital Object Identifier (DOI)
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