Inactivation of nucleolin leads to nucleolar disruption, cell cycle arrest and defects in centrosome duplication
2007
Nucleolin's Role in Cell Division and Centrosome Duplication
publication
Evidence: high
Author Information
Author(s): Ugrinova Iva, Monier Karine, Ivaldi Corinne, Thiry Marc, Storck Sébastien, Mongelard Fabien, Bouvet Philippe
Primary Institution: University of Lyon, Ecole Normale Supérieure de Lyon
Hypothesis
What is the role of nucleolin in cell proliferation and cell cycle regulation?
Conclusion
Nucleolin is essential for proper cell division and centrosome duplication.
Supporting Evidence
- Nucleolin depletion leads to cell growth arrest and increased apoptosis.
- Cells lacking nucleolin showed defects in centrosome duplication.
- Nucleolin is required for the formation of nucleolar structures.
- Depletion of nucleolin resulted in significant changes in cell cycle distribution.
- Cells with low nucleolin levels exhibited increased nuclear alterations.
Takeaway
Nucleolin helps cells grow and divide properly, and without it, cells can get confused and make too many centrosomes.
Methodology
The study used siRNA to knock down nucleolin expression in HeLa cells and primary human fibroblasts to observe effects on cell proliferation and cell cycle.
Participant Demographics
HeLa cells and primary human fibroblasts were used in the study.
Digital Object Identifier (DOI)
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