Validation of Pooled Antibiotic Susceptibility Testing for Urinary Tract Infections
Author Information
Author(s): Haley Emery, Cockerill Frank R., Pesano Rick L., Festa Richard A., Luke Natalie, Mathur Mohit, Chen Xiaofei, Havrilla Jim, Baunoch David
Primary Institution: Pathnostics
Hypothesis
The study aimed to validate a novel susceptibility assay for complicated and recurrent urinary tract infections (UTIs): pooled antibiotic susceptibility testing (P-AST).
Conclusion
The P-AST assay methodology is validated within acceptable parameters when compared to broth microdilution and disk diffusion using CLSI criteria.
Supporting Evidence
- The P-AST method showed essential agreement of ≥90% and very major errors of <1.5%.
- The majority of errors were minor, with essential agreement.
- Very major and major error rates for P-AST decreased to <1.0% when heteroresistance was accounted for.
- The study included monomicrobial urine specimens from 250 subjects.
Takeaway
This study tested a new method for checking how bacteria respond to antibiotics in urine samples, and it worked well compared to traditional methods.
Methodology
The study analyzed urine specimens using standard urine culture and multiplex PCR for microbial identification, followed by antibiotic susceptibility testing using P-AST and standard methods.
Potential Biases
The study design favored standard urine culture by excluding negative and mixed flora cases.
Limitations
The study was limited to monomicrobial cases, and future studies will need to evaluate the performance of P-AST in polymicrobial specimens.
Participant Demographics
Subjects ranged in age from 28.0 to 100.5 years, with a median age of 74.9 years; 54.8% were female and 45.2% were male.
Digital Object Identifier (DOI)
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