How IL-10 Helps Heart Cells Survive Damage
Author Information
Author(s): Sanjiv Dhingra, Ashim K. Bagchi, Ana L. Sharma, Anita K. Singal, Pawan K. Ludke
Primary Institution: University of Manitoba, Winnipeg, Canada
Hypothesis
The study investigates the role of Akt and Jak/Stat pathway in the IL-10 modulation of TNF-α induced cardiomyocyte apoptosis.
Conclusion
IL-10 modulation of TNF-α induced cardiomyocyte apoptosis is mediated by Akt via Stat3 activation.
Supporting Evidence
- IL-10 treatment prevented TNF-α induced apoptosis in cardiomyocytes.
- IL-10 increased Akt levels and Stat3 phosphorylation in cardiomyocytes.
- Inhibition of Akt or Stat3 blocked the protective effects of IL-10.
Takeaway
This study shows that a protein called IL-10 helps heart cells survive when they are damaged by another protein called TNF-α.
Methodology
Cardiomyocytes from adult Sprague Dawley rats were treated with TNF-α and IL-10, and apoptosis was measured using flow cytometry and TUNEL assay.
Limitations
The study was conducted in vitro, which may not fully represent in vivo conditions.
Participant Demographics
Adult male Sprague Dawley rats (250–300 g)
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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