Gene Expression Changes in GABAA Receptors and Cognition Following Chronic Ketamine Administration in Mice
2011

Effects of Long-Term Ketamine Use on Memory in Mice

Sample size: 24 publication 10 minutes Evidence: moderate

Author Information

Author(s): Tan Sijie, Rudd John A., Yew David T.

Primary Institution: The Chinese University of Hong Kong

Hypothesis

Chronic ketamine administration affects learning and memory and alters gene expression levels in the brain.

Conclusion

Chronic exposure to ketamine impairs working memory in mice, potentially due to the up-regulation of Gabra5 subunits in the prefrontal cortex.

Supporting Evidence

  • Mice treated with ketamine took longer to find the platform in the Morris water maze compared to controls.
  • Gene expression analysis showed significant up-regulation of Gabra5 in the prefrontal cortex after ketamine treatment.
  • The study found 110 genes up-regulated and 136 down-regulated in ketamine-treated mice.

Takeaway

Giving mice ketamine for a long time made it harder for them to remember things, and this might be because a specific brain protein increased.

Methodology

Mice were given ketamine or saline for 1 and 3 months, and their memory was tested using the Morris water maze while gene expression was analyzed using microarray and PCR.

Limitations

The study was conducted on mice, which may not fully represent human responses to ketamine.

Participant Demographics

One-month old male ICR mice were used in the study.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0021328

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