The Diagnostic Value of circFBXW7, circABCB10, and circ0103552 Levels in Breast Cancer
Author Information
Author(s): İlhan Burak, Ender Şenol, Kılıç Berkay, Üçüncü Muhammed, Serilmez Murat, Tilgen Yasasever Ceren, Oğuz Soydinç Hilal, Kuras Sibel, Erdoğan Bekir, Alsaadoni Hani, Karanlık Hasan, Bademler Süleyman, Boussios Stergios
Primary Institution: Istanbul University
Hypothesis
This study aimed to determine serum circFBXW7, circABCB10, and circ0103552 levels and compare BC patients and healthy controls to investigate their roles in the molecular mechanism of BC and the significance of these circRNAs in BC diagnosis.
Conclusion
The significant downregulation of circFBXW7 and circ0103552 and the upregulation of circABCB10 are directly related to the diagnosis of breast cancer.
Supporting Evidence
- High ΔCt values of circFBXW7 and circ0103552 and low ΔCt values of circABCB10 were correlated with BC diagnosis.
- CircFBXW7 showed low expression with a Fold Change value of 0.30.
- CircABCB10 showed high expression with a Fold Change value of 11.09.
- Circ0103552 had a positive predictive value of 88.9% when the cut-off value is 6.30.
- The area under the curve (AUC) reached up to 90.8% when circRNAs were used in combination.
Takeaway
This study found that certain circular RNAs in the blood can help doctors diagnose breast cancer more accurately.
Methodology
The study involved 123 individuals, including 92 breast cancer patients and 31 healthy controls, with RNA isolated from serum samples and analyzed using qRT-PCR.
Potential Biases
The patient and control groups may not have been homogeneously distributed regarding patient-specific risk factors.
Limitations
The study had a small sample size and was conducted at a single center, which may limit the generalizability of the findings.
Participant Demographics
58 individuals aged <50 years and 65 aged ≥50 years; 46 premenopausal and 77 postmenopausal women.
Statistical Information
P-Value
p = 0.001 for circABCB10, p < 0.001 for circ0103552, p = 0.043 for circFBXW7
Confidence Interval
CI: 80.3–97.8%
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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