Investigation of Hyalomma turanicum and Hyalomma asiaticum in Pakistan, with notes on the detection of tickborne Rickettsiales
2024

Study of Ticks and Associated Bacteria in Pakistan

Sample size: 314 publication 10 minutes Evidence: moderate

Author Information

Author(s): Ullah Zafar, Liaqat Iram, Khan Mehran, Alouffi Abdulaziz, Almutairi Mashal M., Apanaskevich Dmitry A., Tanaka Tetsuya, Ali Abid

Primary Institution: Government College University, Lahore, Pakistan

Hypothesis

Are Hyalomma turanicum and Hyalomma asiaticum associated with Rickettsia, Anaplasma, and Ehrlichia species in Balochistan, Pakistan?

Conclusion

This study provides the first molecular characterization of Hyalomma turanicum and Hyalomma asiaticum in Balochistan, Pakistan, and identifies associated Rickettsia, Anaplasma, and Ehrlichia species.

Supporting Evidence

  • 314 ticks were collected from 74 hosts, showing a 63.2% infestation rate.
  • 100% identity of Hy. turanicum sequences with previously reported species from Pakistan.
  • Detection of R. aeschlimannii in Hy. turanicum ticks.
  • Coinfection of R. aeschlimannii, Anaplasma sp., and Ehrlichia sp. was observed in 2.5% of Hy. turanicum ticks.

Takeaway

Researchers found two types of ticks in Pakistan that can carry germs that make animals and people sick.

Methodology

Ticks were collected from goats and sheep, identified morphologically, and analyzed using DNA extraction and PCR to detect associated bacteria.

Limitations

The study is limited to a small sample size and specific geographic area in Balochistan.

Participant Demographics

Ticks were collected from 74 hosts, including goats and sheep.

Statistical Information

P-Value

0.27

Digital Object Identifier (DOI)

10.3389/fvets.2024.1500930

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