Study of Extracellular Particles in Human and Canine Blood Samples
Author Information
Author(s): Korenjak Boštjan, Tratenšek Armando, Arko Matevž, Romolo Anna, Hočevar Matej, Kisovec Matic, Berry Maxence, Bedina Zavec Apolonija, Drobne David, Vovk Tomaž, Iglič Aleš, Nemec Svete Alenka, Erjavec Vladimira, Kralj-Iglič Veronika
Primary Institution: University of Ljubljana, Faculty of Health Sciences
Hypothesis
Can interferometric light microscopy (ILM) effectively assess the number density and size of extracellular particles in diluted plasma and blood samples from humans and dogs?
Conclusion
The study demonstrates that ILM can accurately measure the number density and size of extracellular particles in minimally processed blood and plasma samples from both humans and dogs.
Supporting Evidence
- The study analyzed 613 human and 163 canine blood samples.
- Highly statistically significant correlations were found between number density and size of extracellular particles.
- Results showed that measuring extracellular particles in minimally processed blood is feasible for large populations.
Takeaway
Scientists looked at tiny particles in blood from people and dogs to see how many there are and how big they are, and they found a good way to measure them without messing up the samples.
Methodology
The study used interferometric light microscopy to analyze blood and plasma samples for number density and hydrodynamic diameter of extracellular particles.
Potential Biases
Potential biases may arise from sample handling and processing, which could alter the composition of extracellular particles.
Limitations
The study's findings may be influenced by the processing methods of blood samples, which can affect the characteristics of extracellular particles.
Participant Demographics
{"human":{"total":613,"gender":{"female":35,"male":39},"age":{"median":46,"interquartile_range":"31-59"}},"canine":{"total":163,"breed":"brachycephalic","condition":"obstructive airway syndrome"}}
Statistical Information
P-Value
p < 10−4
Statistical Significance
p < 10−3
Digital Object Identifier (DOI)
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