Resistance of BSE Prions to Inactivation
Author Information
Author(s): Giles Kurt, Glidden David V., Beckwith Robyn, Seoanes Rose, Peretz David, DeArmond Stephen J., Prusiner Stanley B.
Primary Institution: University of California San Francisco
Hypothesis
How resistant are BSE prions to inactivation compared to other prion strains?
Conclusion
BSE prions are significantly more resistant to inactivation than mouse-passaged 301V prions and other prion strains.
Supporting Evidence
- BSE prions were found to be up to 1,000-fold more resistant to inactivation than 301V prions.
- BSE prions were 10- and 1,000,000-fold more resistant to inactivation than human sCJD and hamster Sc237 prions, respectively.
- Prion inactivation procedures must be validated by bioassay against the specific prion strain intended for use.
Takeaway
BSE prions are really tough to get rid of, much tougher than prions from mice or humans.
Methodology
The study used bioassays in transgenic mice to measure the infectivity of BSE and 301V prions after various inactivation treatments.
Potential Biases
Potential bias in using rodent-passaged prion strains as models for naturally occurring prions.
Limitations
The study primarily used rodent models, which may not fully represent the inactivation characteristics of prions in their natural hosts.
Participant Demographics
Transgenic mice expressing bovine and mouse prion proteins were used.
Statistical Information
P-Value
p<0.001
Confidence Interval
95% confidence intervals reported for various treatments.
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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