How Apoptosis Affects Schistosoma japonicum in Non-Permissive Hosts
Author Information
Author(s): Peng Jinbiao, Gobert Geoffrey N., Hong Yang, Jiang Weibin, Han Hongxiao, McManus Donald P., Wang Xinzhi, Liu Jinming, Fu Zhiqiang, Shi Yaojun, Lin Jiaojiao
Primary Institution: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences
Hypothesis
The study investigates how apoptosis influences the elimination of Schistosoma japonicum in the non-permissive host Microtus fortis.
Conclusion
The study found that schistosomula from Microtus fortis exhibited significant growth retardation and higher levels of apoptosis compared to those from BALB/c mice.
Supporting Evidence
- Schistosomula from Microtus fortis showed significant growth retardation compared to those from BALB/c mice.
- Apoptosis levels were significantly higher in schistosomula from Microtus fortis.
- Microarray analysis revealed 3,293 down-regulated and 71 up-regulated genes in schistosomula from Microtus fortis.
- Caspase activity was higher in schistosomula from Microtus fortis than in those from BALB/c mice.
- Ultrastructural differences were observed between schistosomula from the two host species.
- Gene expression profiles indicated significant differences in metabolic and developmental pathways.
- Apoptosis-associated genes were differentially expressed in schistosomula from the two hosts.
- Flow cytometry analysis confirmed higher percentages of apoptotic cells in schistosomula from Microtus fortis.
Takeaway
This study shows that a type of cell death called apoptosis helps stop a parasite from growing in certain animals, like the reed vole.
Methodology
The study used electron microscopy, microarray analysis, and various assays to compare schistosomula from two different host species.
Limitations
The study primarily focuses on two host species and may not generalize to other hosts or conditions.
Participant Demographics
The study involved specific-pathogen free female Microtus fortis and BALB/c mice.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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