Virus Detection Using Random Multiplex PCR
Author Information
Author(s): Clem Amy L, Sims Jonathan, Telang Sucheta, Eaton John W, Chesney Jason
Primary Institution: University of Louisville, Kentucky, USA
Hypothesis
Random amplification of viral nucleic acids isolated from human plasma might provide a powerful new method to detect and identify novel and recombined viruses.
Conclusion
The study suggests that random multiplex (RT)-PCR can detect and identify multiple viruses in human plasma without using virus-specific reagents.
Supporting Evidence
- The method detected Adenovirus Type 17, Coxsackievirus A7, and Respiratory Syncytial Virus B in human plasma.
- The random multiplex PCR method is sensitive to ~1000 genome equivalents/ml.
- The study demonstrated the ability to identify viruses without using virus-specific reagents.
Takeaway
Scientists found a new way to quickly find viruses in blood by using a special test that doesn't need to know the virus's exact makeup first.
Methodology
The study used a novel PCR method called Random Multiplex (RT)-PCR to amplify viral nucleic acids from human plasma after filtration and nuclease treatment.
Limitations
The method's sensitivity is lower than virus-specific PCR, and there may be background amplification leading to false positives.
Digital Object Identifier (DOI)
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