Surface expression and limited proteolysis of ADAM10 are increased by a dominant negative inhibitor of dynamin
2011
Inhibition of Dynamin Increases ADAM10 Surface Expression and Proteolysis
Sample size: 5
publication
10 minutes
Evidence: moderate
Author Information
Author(s): Carey Robyn M, Blusztajn Jan K, Slack Barbara E
Primary Institution: Boston University School of Medicine
Hypothesis
Inhibition of dynamin function might be the mechanism underlying kinase-dependent sAPPĪ± release.
Conclusion
Inhibition of dynamin-dependent endocytosis increases surface expression of ADAM10 and promotes its proteolysis.
Supporting Evidence
- Inhibition of dynamin function increases surface expression of both mature and immature forms of ADAM10.
- Surface levels of ADAM10 were significantly increased in cells co-expressing ADAM10 and the dynamin mutant.
- Activation of muscarinic M3 receptors did not affect ADAM10 surface expression.
Takeaway
When a certain protein's internalization is blocked, it stays on the cell surface longer and gets broken down more, which can change how cells behave.
Methodology
HEK cells were transfected with ADAM10 and a dominant negative dynamin mutant, and surface expression was analyzed by Western blotting.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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