CORE: A Phylogenetically-Curated 16S rDNA Database of the Core Oral Microbiome
Author Information
Author(s): Griffen Ann L., Beall Clifford J., Firestone Noah D., Gross Erin L., DiFranco James M., Hardman Jori H., Vriesendorp Bastienne, Faust Russell A., Janies Daniel A., Leys Eugene J.
Primary Institution: The Ohio State University
Hypothesis
The CORE database aims to provide a comprehensive and minimally redundant representation of the bacteria that regularly reside in the human oral cavity.
Conclusion
The CORE database outperformed other public databases in identifying human oral bacterial 16S sequences.
Supporting Evidence
- CORE provided improved performance for identifying human oral bacterial sequences compared to other databases.
- The database was constructed using a comprehensive set of sequences from various studies.
- CORE was designed to minimize redundancy and improve taxonomic accuracy.
- Statistical support for taxonomic divisions was based on maximum-likelihood topology and bootstrap values.
Takeaway
Researchers created a special database to help identify bacteria in our mouths better, which is important for understanding oral health.
Methodology
The study involved developing a 16S rDNA database by curating sequences from published surveys and comparing its performance against other databases using BLAST.
Potential Biases
Potential biases may arise from the reliance on existing databases for sequence classification.
Limitations
The database may still contain some misclassifications and ambiguities due to the nature of 16S sequencing.
Participant Demographics
The study included sequences derived from human dental plaque and pharyngeal samples from 200 different human subjects.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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