Proximal genomic localization of STAT1 binding and regulated transcriptional activity
2006
STAT1 Binding and Gene Activity in Response to Interferon-gamma
publication
Evidence: moderate
Author Information
Author(s): Samuel Wormald, Douglas J. Hilton, Gordon K. Smyth, Terence P. Speed
Primary Institution: The Walter and Eliza Hall Institute of Medical Research
Hypothesis
How does STAT1 binding correlate with transcriptional activity in response to interferon-gamma?
Conclusion
STAT1 binds closely to regions of the genome that are actively transcribing mRNA in response to interferon-gamma.
Supporting Evidence
- STAT1 binding is closely associated with increased mRNA transcription in response to IFN-γ.
- The study found that STAT1 binding sites are often located near the start of genes that respond to IFN-γ.
- There is a high degree of overlap between STAT1 and STAT2 binding sites in response to both IFN-γ and IFN-α.
Takeaway
When a special protein called STAT1 attaches to certain parts of our DNA, it helps turn on the genes that fight infections.
Methodology
The study used tiling microarray and chromatin immunoprecipitation microarray (ChIP-chip) experiments to analyze STAT1 binding and transcriptional activity.
Digital Object Identifier (DOI)
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