Osteopontin is required for full expression of the transformed phenotype by the ras oncogene
2000
Osteopontin's Role in Cancer Transformation
publication
Evidence: moderate
Author Information
Author(s): Wu Y, Denhardt D T, Rittling S R
Primary Institution: Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ, USA
Hypothesis
Is osteopontin required for the full expression of the transformed phenotype by the ras oncogene?
Conclusion
Osteopontin is necessary for maximal transformation by the ras oncogene, as its absence slows tumor formation.
Supporting Evidence
- Osteopontin is associated with neoplastic transformation based on expression patterns.
- Ras-transformed cell lines from both wildtype and OPN-deficient mice could form colonies in soft agar.
- OPN-deficient cell lines formed tumors much more slowly than wildtype cells in mice.
Takeaway
Osteopontin helps cancer cells grow and form tumors, and without it, they grow more slowly.
Methodology
The study used 3T3 cells from wildtype and OPN-deficient mice, transformed by oncogenic ras, to assess OPN's role in transformation and tumorigenesis.
Digital Object Identifier (DOI)
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