How a Distant Enhancer Regulates the PADI3 Gene in Skin Cells
Author Information
Author(s): Chavanas Stéphane, Adoue Véronique, Méchin Marie-Claire, Ying Shibo, Dong Sijun, Duplan Hélène, Charveron Marie, Takahara Hidenari, Serre Guy, Simon Michel
Primary Institution: UMR 5165, CNRS-Toulouse III University, CHU Purpan, Toulouse, France
Hypothesis
The study investigates the role of a long-range enhancer in regulating the expression of the PADI3 gene in differentiated keratinocytes.
Conclusion
The study found that a distant enhancer, named PIE, significantly increases the expression of the PADI3 gene in response to calcium stimulation in keratinocytes.
Supporting Evidence
- The enhancer PIE is located 86-kb from the PADI3 promoter and is essential for its regulation.
- Calcium stimulation increases the physical proximity between PIE and the PADI3 promoter.
- AP-1 factors c-Jun and c-Fos are required for the enhancer activity of PIE.
Takeaway
Scientists discovered a special DNA segment far from the PADI3 gene that helps control how much of the gene is used in skin cells, especially when the cells are told to grow up.
Methodology
The researchers used cell culture, luciferase assays, and chromatin immunoprecipitation to study the enhancer's effects on gene expression.
Limitations
The study does not rule out potential non-specific effects of the AP-1 inhibitor used.
Participant Demographics
Normal human epidermal keratinocytes were used in the experiments.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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