Effects of Alnus japonica Extract and Oregonin on Muscle Loss
Author Information
Author(s): An Da Hyeon, Lee Chan Ho, Kwon Yeeun, Kim Tae Hee, Kim Eun Ji, Jung Jae In, Min Sangil, Cheong Eun Ju, Kim Sohyun, Kim Hee Kyu, Choi Sun Eun, Chen Chung-Yi, Enioutina Elena Y.
Primary Institution: Kangwon National University
Hypothesis
Alnus japonica extract and its active compound, oregonin, can promote muscle protein synthesis and inhibit its degradation in myofibroblasts through antioxidant activity and by inhibiting myofibroblast apoptosis.
Conclusion
Alnus japonica extract and oregonin effectively prevent muscle cell apoptosis, promote muscle protein synthesis, and inhibit muscle protein degradation in vitro.
Supporting Evidence
- AJHW and oregonin significantly increased cell viability and reduced apoptosis.
- AJHW and oregonin enhanced muscle protein synthesis through the upregulation of MyoD and Myogenin.
- AJHW and oregonin diminished muscle degradation by downregulating Atrogin-1 and MuRF1.
- The activation of the Akt/mTOR pathway and inhibition of the FoxO3α pathway were observed.
Takeaway
This study found that a natural extract from a plant called Alnus japonica can help muscles stay strong and healthy by stopping cell death and helping muscles grow.
Methodology
C2C12 cells were treated with H2O2 and dexamethasone to induce muscle loss, followed by administration of AJHW and oregonin to assess their effects on cell viability, apoptosis, and muscle protein synthesis.
Limitations
The study did not clearly elucidate the molecular mechanisms related to the intracellular effects of oregonin.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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