Measuring N-Acetyl-L-Cysteine and N-(2-Mercaptopropionyl)-Glycine in Medicines
Author Information
Author(s): Lea Kukoc-Modun, Njegomir Radić
Primary Institution: Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split
Hypothesis
A simple and cost-effective spectrophotometric method can accurately determine the concentrations of NAC and MPG in pharmaceutical preparations.
Conclusion
The proposed spectrophotometric method is accurate and reliable for determining NAC and MPG in pharmaceutical formulations without interference from common excipients.
Supporting Evidence
- The method showed a good correlation coefficient of 0.9999.
- The detection limits were 0.14 μM for NAC and 0.13 μM for MPG.
- The recovery of added NAC or MPG was between 98% and 102%.
Takeaway
Scientists created a new way to measure two important medicines using light, which is simple and doesn't get confused by other ingredients.
Methodology
The method involves a coupled redox-complexation reaction using Fe(III) and TPTZ to measure NAC and MPG concentrations.
Limitations
The method may not be applicable for samples with interfering substances that have higher reducing potentials.
Statistical Information
Statistical Significance
p>0.1
Digital Object Identifier (DOI)
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