Antioxidants Reduce Antibiotic Resistance in Pseudomonas aeruginosa Biofilms
Author Information
Author(s): Higazy Doaa, Ahmed Marwa N., Ciofu Oana
Primary Institution: Costerton Biofilm Center, Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark
Hypothesis
Can antioxidants like N-acetyl-cysteine and Edaravone reduce the development of antibiotic resistance in Pseudomonas aeruginosa biofilms exposed to ciprofloxacin?
Conclusion
The study found that combining ciprofloxacin with antioxidants significantly reduced the development of antibiotic resistance in Pseudomonas aeruginosa biofilms.
Supporting Evidence
- Combining ciprofloxacin with antioxidants effectively reduced the development of ciprofloxacin resistance.
- Isolates from biofilms treated with ciprofloxacin and antioxidants had significantly lower minimum inhibitory concentrations of ciprofloxacin.
- Whole-genome sequencing revealed fewer mutations in resistance-related genes in biofilms treated with antioxidants.
Takeaway
This study shows that adding certain antioxidants to antibiotics can help stop bacteria from becoming resistant, making the antibiotics work better.
Methodology
In vitro experimental evolution studies were conducted using flow cells and glass beads biofilm models.
Limitations
The effects of antioxidants varied depending on the specific antioxidant and the bacterial growth form.
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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