Genome Sequence of Bacteroides Fragilis Phage ATCC 51477-B1
Author Information
Author(s): Shawn A Hawkins, Alice C Layton, Steven Ripp, Dan Williams, Gary S Sayler
Primary Institution: The University of Tennessee, Knoxville, TN, USA
Hypothesis
The genome of phage ATCC 51477-B1 was sequenced to promote fecal source tracking assay development.
Conclusion
The genome of phage ATCC 51477-B1 was successfully sequenced, revealing important genetic information for fecal pollution tracking.
Supporting Evidence
- Bacteroides phages are more abundant in fecal matter than E. coli.
- The genome was found to contain 46 putative open reading frames.
- The phage genome was assembled with 91% similarity for 100× coverage.
Takeaway
Scientists figured out the genetic code of a virus that can help track pollution from poop in water.
Methodology
The phage was propagated on B. fragilis HSP40 and its genome was sequenced using various methods including PCR and pyrosequencing.
Limitations
The study is limited by the difficulty in detecting Bacteroides phages due to the need for anaerobic cultivation.
Digital Object Identifier (DOI)
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