Endophyte Microbiome Diversity in Micropropagated Atriplex canescens and Atriplex torreyi var griffithsii
2011
Diversity of Microbial Communities in Atriplex Plants
Sample size: 90
publication
Evidence: moderate
Author Information
Author(s): Mary E. Lucero, Adrian Unc, Peter Cooke, Scot Dowd, Shulei Sun
Primary Institution: New Mexico State University
Hypothesis
The study aims to explore the diversity of systemic endophytes in micropropagated Atriplex species.
Conclusion
Micropropagated Atriplex retains a complex microbiome that includes diverse strains capable of influencing host physiology.
Supporting Evidence
- Light, electron, and confocal microscopy revealed microbial cells in aseptically regenerated leaves and roots.
- Clone libraries and tag-encoded FLX amplicon pyrosequencing revealed diverse fungal and bacterial taxa.
- Micropropagated Atriplex retains a complex microbiome which includes diverse strains.
Takeaway
This study found that plants called Atriplex have many tiny living things, like bacteria and fungi, that help them grow and stay healthy.
Methodology
The study used microscopy, isolate culturing, and sequencing to assess microbial diversity.
Limitations
Primer biases limited the recovery of diverse sequences from complex communities.
Digital Object Identifier (DOI)
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