The Netrin-related domain of Sfrp1 interacts with Wnt ligands and antagonizes their activity in the anterior neural plate
2008
Sfrp1's Netrin-related Domain Interacts with Wnt Ligands
publication
Evidence: moderate
Author Information
Author(s): Lopez-Rios Javier, Esteve Pilar, Ruiz Jose Maria, Bovolenta Paola
Primary Institution: Instituto Cajal, CSIC, Madrid, Spain
Hypothesis
Does the Netrin-related domain of Sfrp1 interact with Wnt ligands and affect their activity?
Conclusion
The Netrin-related domain of Sfrp1 can mimic the full protein's function by binding to Wnt8 and inhibiting Wnt canonical signaling.
Supporting Evidence
- Sfrp1NTR mimics the function of the full-length Sfrp1.
- Sfrp1CRD does not replicate the effects of Sfrp1 over-expression.
- Sfrp1NTR binds to Wnt8 and prevents Wnt canonical signaling activation.
Takeaway
Sfrp1 has two parts, and one part helps it stop Wnt signals, which are important for eye development in fish.
Methodology
The study used biochemical studies, mutational analysis, and functional assays in cell culture and medaka-fish embryos.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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