Genetic Diversity of Dengue Virus in the Philippines (2014–2019)
Author Information
Author(s): Velasco John Mark, Klungthong Chonticha, Chinnawirotpisan Piyawan, Diones Paula Corazon, Valderama Maria Theresa, Leonardia Susie, Manasatienkij Wudtichai, Joonlasak Khajohn, Rodpradit Prinyada, Mateo Jennifer, Vila Vicente II, Navarro Fatima Claire, Jones Anthony, Farmer Aaron, Fernandez Stefan
Primary Institution: Department of Virology, WRAIR-AFRIMS, Bangkok, Thailand
Hypothesis
What is the genetic diversity of dengue virus circulating in the Philippines and how does it compare with dengue vaccine strains?
Conclusion
The study found low genotype complexity and genetically distinct clades of dengue virus circulating in the Philippines from 2014 to 2019.
Supporting Evidence
- Dengue virus has four distinct serotypes, each with significant genetic diversity.
- The study analyzed 127 samples from patients diagnosed with dengue.
- Phylogenetic analysis revealed distinct genotypes for each serotype.
- Low genotype complexity was observed among circulating dengue viruses.
- Comparison with vaccine strains showed significant amino acid changes.
- Serotype shifts were noted in the years studied.
- Vaccination campaigns may have influenced the genetic diversity of circulating strains.
- Further research is needed to understand the implications for vaccine efficacy.
Takeaway
Dengue virus has different types, and scientists studied how these types changed in the Philippines over several years to see if they matched with vaccine types.
Methodology
Demographic data and blood samples were collected from dengue patients, and RT-PCR was used to confirm infections followed by whole genome sequencing.
Limitations
The study was limited to a six-year period and did not cover all geographic areas of the Philippines, which may have missed rare genotypes.
Participant Demographics
Patients diagnosed with dengue from various regions in the Philippines.
Digital Object Identifier (DOI)
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