CD24's Role in Tight Junctions and Barrier Function in Oral Epithelial Cells
Author Information
Author(s): Ye Ping, Nadkarni Mangala A, Simonian Mary, Hunter Neil
Primary Institution: Institute of Dental Research, Westmead Millennium Institute and Westmead Centre for Oral Health, Westmead Hospital, Westmead, NSW, Australia
Hypothesis
CD24 regulates the expression of tight junction proteins and affects barrier function in oral epithelial monolayers.
Conclusion
CD24 is crucial for maintaining the expression of tight junction proteins, which enhances the barrier function of oral epithelial cells.
Supporting Evidence
- CD24 is strongly expressed in the epithelial attachment to the tooth and in the lining of periodontal pockets.
- Blocking CD24 with antibodies reduced the passage of low molecular weight dextran across epithelial monolayers.
- Silencing CD24 mRNA increased permeability of the epithelial monolayer to dextran.
- Gene expression analysis showed significant up-regulation of tight junction proteins in response to anti-CD24 treatment.
Takeaway
CD24 helps keep the connections between cells strong, which stops bad stuff from getting through in the mouth.
Methodology
The study used H413 oral epithelial cell monolayers to assess barrier function and gene expression changes in response to anti-CD24 peptide antibody treatment.
Statistical Information
P-Value
< 0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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