Analysis of Tumor Infiltrating Lymphocytes in Breast Cancer
Author Information
Author(s): P. Whitford, E.A. Mallon, W.D. George, A.M. Campbell
Primary Institution: University of Glasgow
Hypothesis
What is the phenotype and activation status of tumor infiltrating lymphocytes in breast cancer?
Conclusion
The study found that tumor infiltrating lymphocytes predominantly consist of CD8+ T cells, and their levels inversely correlate with estrogen receptor levels in the tumor.
Supporting Evidence
- CD8+ T cells were found in greater numbers than CD4+ T cells in most patients.
- There was an inverse correlation between lymphocytic infiltration and estrogen receptor levels.
- Both CD8+ and CD4+ T cells had significantly higher activation markers compared to peripheral blood.
Takeaway
In breast cancer, the immune cells that invade the tumor are mostly a type called CD8+ T cells, and more of these cells are found when the tumor has lower estrogen receptors.
Methodology
Flow cytometry was used to analyze the phenotype and activation markers of tumor infiltrating lymphocytes from tumor and blood samples of patients.
Limitations
Some tumors had too few lymphocytes for analysis, and the study did not separate tumor infiltrating lymphocytes from tumor cells.
Participant Demographics
Patients undergoing definitive surgery for breast carcinoma, with a mix of tumor grades and stages.
Statistical Information
P-Value
p<0.01, p<0.001, p=0.034, p=0.029
Statistical Significance
p<0.01
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