Identification of the hASCT2-binding domain of the Env ERVWE1/syncytin-1 fusogenic glycoprotein
2006
Identifying the Binding Domain of Syncytin-1 Protein
publication
Evidence: moderate
Author Information
Author(s): Cheynet Valérie, Oriol Guy, Mallet François
Primary Institution: UMR 2714 CNRS-bioMérieux, IFR128 BioSciences Lyon-Gerland, Ecole Normale Supérieure de Lyon, France
Hypothesis
What is the minimal receptor-binding domain of the HERV-W envelope protein syncytin-1?
Conclusion
The study identified a 124 amino acid region of the syncytin-1 protein as the minimal domain necessary for interaction with the hASCT2 receptor.
Supporting Evidence
- The HERV-W envelope protein syncytin-1 is involved in the formation of the placental syncytiotrophoblast layer.
- Syncytin-1-induced cell-cell fusion depends on interaction with the hASCT2 receptor.
- A 124 residue region of the mature SU was determined as the minimal receptor-binding domain.
- The study confirmed that soluble SU protein behaves similarly to the native syncytin-1 protein.
Takeaway
Scientists found a small part of a protein that helps cells stick together in the placenta, which is important for pregnancy.
Methodology
An in vitro binding assay was designed to evaluate the interaction of HERV-W envelope-derived soluble SU domains with the hASCT2 receptor.
Digital Object Identifier (DOI)
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